Source Name: 2021_Wang
Literature Information
| Literature Title | Effect of Flexibility, Lipophilicity, and the Location of Polar Residues on the Passive Membrane Permeability of a Series of Cyclic Decapeptides |
| Doi | 10.1021/acs.jmedchem.1c00775 |
| Research Group |
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| Data Number | 24 |
| Minimum Molecular Weight | 959.2 |
| Maximum Molecular Weight | 1169.5 |
Assay Information 1
| Assay Type | PAMPA |
| Permeability Type | logPe |
| Membrane Measurement Direction (Exclude PAMPA) | - |
| Assay Detail | PAMPA was used as a primary screen to predict gastrointestinal permeability. The assay was carried out in 96-well plates by measuring the ability of the compound to diffuse from a donor to an acceptor plate separated by a 9-10 μm hexadecane liquid layer coated on a polycarbonate filter plate. To minimize solubility issues, compounds were loaded at 5 μm in the donor compartment and the assay buffer contained 5% DMSO. The permeability was derived from the compound concentration measured by LC-MS/MS in the acceptor compartment after a 4 h incubation time. |
Assay Information 2
| Assay Type | - |
| Permeability Type | - |
| Membrane Measurement Direction (Exclude PAMPA) | - |
| Assay Detail | - |