| Assay Detail |
Compounds were quantified using an LC/MS assay. Low resolution mass spectra (ESI) were collected on a Shimadzu UPLC & API 4000 (AB SCIEX, USA). Compounds were resolved on a Phenomenex Luna C18 (2.0 x 50 mm^2, 5 μm) column at room temperature with a flow of 0.5mL/min. The gradient consisted of eluents A (0.1% formic acid in double distilled water) and B (0.1% formic acid in HPLC-grade acetonitrile). The gradient method started at 5% of B for 0.5 minutes and then followed a linear gradient to 100% B for 1.3 minutes. The column was then washed with 95% B for 0.4 minutes, brought back down to 5% B in 0.1 minutes, and equilibrated at 5% B for an additional 0.7 minutes.
Test Concentration: 10 μM, Lipid Membrane Composition: 1.8% (w/v) of lecithin in dodecane, Aqueous Solution: Phosphate buffer solution (10 mM, pH 7.4), Incubation Temperature: R.T., Incubation Time & Condition: 16 h; 150 rpm, DMSO%: 0.1%, Cosolvent: PBS.
|