| Assay Detail |
Analysis of all individually synthesized compounds by the established Caco-2 assay was outsourced to Cyprotex. For specific details of the assay conditions, interested parties should contact Cyprotex directly. In general, after a 20Aday cell culture of Caco-2 cells, individual compounds were were incubated at 10 µM for 2 h and 37 °C, after which concentrations in apical and basolateral compartments were quantified by LCAMS/MS to calculate compound flux in AAtoAB direction. Lucifer Yellow was used to validate membrane integrity of the Caco-2 cell monolayer and Atenolol, Propranolol, and Talinolol were used as controls with known Caco-2 permeability.
|